| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pendleton, Zackary A (10)
|
NCGFSC |
32.46 |
34.60 |
2.14 |
| 2 |
Da Silva, Nicholas S (10)
|
NCASTF |
36.43 |
34.95 |
-1.48 |
| 3 |
Kolinski, William W (10)
|
NCTAC |
35.26 |
35.00 |
-0.26 |
| 4 |
Ju, Joshua E (10)
|
NCTAC |
40.15 |
35.76 |
-4.39 |
| 5 |
Suphavadeprasit, Arman (10)
|
NCTAC |
36.62 |
35.92 |
-0.70 |
| 6 |
Rothchild, Brennan T (10)
|
NCTAC |
35.37 |
36.20 |
0.83 |
| 7 |
Dunaway, Jake B (8)
|
NCTAC |
38.47 |
38.38 |
-0.09 |
| 8 |
Peng, Alistair (7)
|
NCTAC |
40.15 |
38.94 |
-1.21 |
| 9 |
Kim, Lucas B (8)
|
NCTAC |
37.03 |
39.31 |
2.28 |
| 10 |
Bacon, Chance M (8)
|
NCGFSC |
42.58 |
41.37 |
-1.21 |
| 11 |
Hignite, Ian G (9)
|
NCTAC |
40.53 |
42.47 |
1.94 |
| 12 |
Collins, Blake D (9)
|
NCGFSC |
43.88 |
43.23 |
-0.65 |
| 13 |
Lilyquist, Mikael P (9)
|
NCTAC |
45.62 |
43.41 |
-2.21 |
| 14 |
Dettman, Jonah H (9)
|
NCGFSC |
55.12 |
43.51 |
-11.61 |
| 15 |
March, Brady A (7)
|
NCTAC |
44.01 |
44.22 |
0.21 |
| 16 |
Everhart, Jackson G (8)
|
NCASTF |
46.99 |
44.98 |
-2.01 |
| 17 |
Brown, Jayden M (8)
|
NCTAC |
52.79 |
50.93 |
-1.86 |
| 18 |
Radzai, Tye B (6)
|
NCGFSC |
1:16.13 |
1:06.49 |
-9.64 |
| 19 |
Smith, Jeremiah C (7)
|
NCGFSC |
1:02.70 |
1:07.25 |
4.55 |