| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gibson, Rachel L (15)
|
NCRACY |
2:41.08 |
2:47.66 |
6.58 |
| 2 |
Ritter, Rachel E (15)
|
NCYBAC |
2:45.32 |
2:47.83 |
2.51 |
| 3 |
Schuster, Ava P (14)
|
NCLOY |
3:12.59 |
3:04.37 |
-8.22 |
| 4 |
Bergelin, Katie E (15)
|
NCTYDE |
|
3:04.59 |
- |
| 5 |
Quinn, Chelsea M (13)
|
NCYBAC |
3:00.56 |
3:04.60 |
4.04 |
| 6 |
Sorjonen, Taika A (16)
|
NCGCY |
2:52.72 |
3:09.02 |
16.30 |
| 7 |
Gleason, Riley E (13)
|
NCRACY |
3:08.86 |
3:12.93 |
4.07 |
| 8 |
Whitworth, Margaret E (13)
|
NCTYDE |
|
3:15.16 |
- |
| 9 |
Morrow, Julianna L (14)
|
NCLOY |
3:18.26 |
3:17.64 |
-0.62 |
| 10 |
Cuccinello, Dakota T (14)
|
NCLOY |
3:33.52 |
3:31.56 |
-1.96 |
| 11 |
Roman, Sophia N (12)
|
NCFAST |
|
3:40.91 |
- |
| 12 |
Lewis, Piper A (13)
|
NCTYDE |
|
3:42.95 |
- |
| 13 |
Hawkins, Abigail R (15)
|
NCTYDE |
3:50.40 |
3:59.50 |
9.10 |