| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fu, Jiayu (16)
|
NCWAVE |
28.89 |
27.23 |
-1.66 |
| 2 |
Fullerton, Mikaela C (17)
|
NCWAVE |
26.96 |
28.73 |
1.77 |
| 3 |
Gooch, Ellen J (18)
|
NCHAC |
28.99 |
29.12 |
0.13 |
| 4 |
Grasso, Aynslee M (16)
|
NCWAVE |
28.47 |
29.16 |
0.69 |
| 5 |
Erickson, Grace A (16)
|
NCHAC |
31.99 |
29.30 |
-2.69 |
| 6 |
Baldwin, LeighAnne N (17)
|
NCWAVE |
31.24 |
29.79 |
-1.45 |
| 7 |
Hurlbert, Katherine R (17)
|
NCWAVE |
27.61 |
30.08 |
2.47 |
| 8 |
Adams, Jennifer A (15)
|
NCWAVE |
27.79 |
30.11 |
2.32 |
| 9 |
Behringer, Ashley M (15)
|
NCHAC |
30.97 |
30.14 |
-0.83 |
| 10 |
Walker, Brooke A (15)
|
NCHAC |
32.99 |
30.86 |
-2.13 |
| 11 |
Kennedy, Carlyle R (15)
|
NCWAVE |
50.65 |
32.96 |
-17.69 |