| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Taylor, Kayana R (14)
|
NCYSST |
33.71 |
32.39 |
-1.32 |
| 2 |
Hernandez-Pena, Valeria (14)
|
NCYSST |
37.57 |
34.25 |
-3.32 |
| 3 |
Parmenter, Katelyn L (13)
|
NCYSST |
37.87 |
34.95 |
-2.92 |
| 4 |
Hubbard, Ashley C (14)
|
SCYCSC |
33.28 |
35.47 |
2.19 |
| 5 |
Hubbard, Alicia R (13)
|
SCYCSC |
1:09.43 |
37.11 |
-32.32 |
| 6 |
Seay, Aleah M (13)
|
NCWNCY |
|
37.74 |
- |
| 7 |
Humphrey, Chloe E (13)
|
SCYCSC |
38.62 |
37.84 |
-0.78 |
| 8 |
McCormack, Olivia A (14)
|
NCWNCY |
39.42 |
38.37 |
-1.05 |
| 10 |
Tippabhatla, Simran (13)
|
SCYCSC |
40.64 |
39.28 |
-1.36 |
| 11 |
Kaczynski, Abigail E (14)
|
SCYCSC |
|
39.86 |
- |
| 12 |
Cattaneo, Courtney A (13)
|
SCYCSC |
43.16 |
40.61 |
-2.55 |
| 13 |
McCarty, Elizabeth M (13)
|
SCYCSC |
|
40.82 |
- |
| 14 |
McGee, Isabella H (13)
|
NCRACY |
46.98 |
41.05 |
-5.93 |
| 15 |
Jolly, Katherine E (13)
|
NCRACY |
|
42.41 |
- |
| 18 |
Gleason, Riley E (13)
|
NCRACY |
|
44.81 |
- |
| 19 |
Tuttle, Mikayla R (13)
|
NCWNCY |
|
47.60 |
- |
|
Shlien-Dellinger, Phoenix S (13)
|
NCWNCY |
38.98 |
NS |
- |