| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Jann, Christian D (18)
|
SCYSSC |
56.54 |
58.44 |
1.90 |
| 3 |
Morrison, Matthew G (17)
|
NCWNCY |
59.19 |
1:00.11 |
0.92 |
| 4 |
Williams, Nathan T (17)
|
SCYCSC |
55.88 |
1:00.19 |
4.31 |
| 5 |
McCosh, Ethan A (15)
|
NCYSST |
57.80 |
1:00.84 |
3.04 |
| 6 |
Procopii, Stephan C (17)
|
NCYSST |
1:00.15 |
1:02.27 |
2.12 |
| 7 |
Talbert, Joshua D (17)
|
NCYSST |
59.03 |
1:02.57 |
3.54 |
| 8 |
Little, James E (16)
|
SCAAYM |
1:02.04 |
1:03.00 |
0.96 |
| 9 |
Kolomiyets, Israel Z (16)
|
NCWNCY |
1:02.60 |
1:03.10 |
0.50 |
| 10 |
Ploplis, Reilly M (17)
|
NCRACY |
1:03.20 |
1:04.34 |
1.14 |
| 11 |
Brown, Daniel T (15)
|
SCYCSC |
1:02.38 |
1:04.97 |
2.59 |
| 13 |
Scott, Andrew B (16)
|
SCYCSC |
1:11.09 |
1:08.52 |
-2.57 |
| 14 |
Guffey, Nathaniel A (15)
|
NCWNCY |
1:07.73 |
1:08.94 |
1.21 |
| 16 |
Webb, William J (16)
|
SCYCSC |
1:13.31 |
1:10.76 |
-2.55 |
| 17 |
MacDermott, Maddux D (15)
|
NCRACY |
1:09.91 |
1:13.07 |
3.16 |