| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Post, Cian D (12)
|
NCWOW |
33.33 |
31.54 |
-1.79 |
| 2 |
Camacho, Clement D (11)
|
NCCFAC |
30.74 |
32.02 |
1.28 |
| 3 |
Marsteiner, Matthew F (12)
|
NCWAVE |
32.99 |
32.29 |
-0.70 |
| 4 |
Brown, Lawson W (11)
|
NCCFAC |
32.44 |
32.91 |
0.47 |
| 5 |
Hutchins, Matthew T (12)
|
NCWAVE |
35.79 |
33.32 |
-2.47 |
| 6 |
Hughes, Jackson C (12)
|
NCWOW |
32.67 |
33.59 |
0.92 |
| 7 |
Witmer, William C (12)
|
NCCFAC |
36.42 |
34.09 |
-2.33 |
| 8 |
Tillery, Wade H (12)
|
NCWOW |
35.86 |
35.62 |
-0.24 |
| 9 |
Reiniche, Ryan J (11)
|
NCWOW |
34.95 |
35.89 |
0.94 |
| 10 |
Hawthorne, Maxwell S (11)
|
NCWOW |
33.57 |
36.26 |
2.69 |
| 11 |
Fary, Elliott K (12)
|
NCCFAC |
37.53 |
36.98 |
-0.55 |
| 12 |
House, William E (11)
|
NCCFAC |
36.29 |
37.73 |
1.44 |
| 13 |
Arredondo, Nathan B (11)
|
NCWOW |
|
38.27 |
- |
| 14 |
Burckhardt, Ayden C (11)
|
NCHCAC |
37.61 |
39.75 |
2.14 |
| 15 |
Page, Logan A (12)
|
NCWAVE |
42.55 |
40.22 |
-2.33 |
| 16 |
Brown, Noah D (12)
|
NCCFAC |
45.04 |
40.80 |
-4.24 |
| 17 |
Mejouev, Maxim (12)
|
NCWOW |
42.09 |
41.12 |
-0.97 |
| 18 |
DiGuiseppe, Julian M (12)
|
NCBAC |
|
42.77 |
- |
| 19 |
Grimshaw, Alexander L (11)
|
NCSSS |
46.60 |
42.92 |
-3.68 |
| 20 |
Moujeahid, Adam K (11)
|
NCWAVE |
45.70 |
43.45 |
-2.25 |
| 21 |
McDonald, Ray D (11)
|
NCCFAC |
51.24 |
44.66 |
-6.58 |
| 22 |
Ausband, Laith J (11)
|
NCCFAC |
|
45.04 |
- |
| 23 |
Jenkins, Owen C (11)
|
NCHCAC |
43.49 |
45.10 |
1.61 |
| 24 |
Eder, Ryker P (11)
|
NCCFAC |
44.80 |
45.73 |
0.93 |
|
Park, Dylan T (11)
|
NCWOW |
41.74 |
NS |
- |