| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Adkins, Charles A (18)
|
NCWOW |
21.52 |
21.42 |
-0.10 |
| 2 |
Winchester, Austin O (16)
|
NCWOW |
21.82 |
21.55 |
-0.27 |
| 3 |
Howard, Finn D (17)
|
NCWOW |
21.31 |
21.92 |
0.61 |
| 4 |
Parker, William H (15)
|
NCCFAC |
22.09 |
22.64 |
0.55 |
| 5 |
Settlemyre, Jeffrey P (17)
|
UNAT |
22.36 |
22.80 |
0.44 |
| 6 |
Furbay, Patrick D (15)
|
NCCFAC |
22.27 |
23.08 |
0.81 |
| 7 |
Davis, Matthew C (16)
|
NCWOW |
23.99 |
23.14 |
-0.85 |
| 8 |
Teeters, Thomas D (16)
|
NCCFAC |
23.51 |
23.43 |
-0.08 |
| 9 |
Page, Benjamin L (13)
|
NCWOW |
|
23.93 |
- |
| 10 |
Butner, Mark L (15)
|
NCWOW |
25.42 |
24.10 |
-1.32 |
| 11 |
Langtry, Robert J (17)
|
NCWOW |
24.49 |
24.30 |
-0.19 |
| 12 |
Spencer, Brandon P (15)
|
NCCFAC |
24.79 |
24.72 |
-0.07 |
| 13 |
Kirby, Evan N (16)
|
NCWOW |
23.83 |
24.73 |
0.90 |
| 14 |
Vitt, Adam J (17)
|
NCWOW |
25.02 |
25.04 |
0.02 |
| 15 |
Dai, Kevin (15)
|
NCWOW |
25.32 |
25.10 |
-0.22 |
| 16 |
Luo, Matthew Z (15)
|
NCWOW |
26.28 |
25.16 |
-1.12 |
| 17 |
Brown, Philip M (13)
|
NCCFAC |
48.96 |
25.44 |
-23.52 |
| 18 |
Huo, Jack X (15)
|
NCCFAC |
25.81 |
25.48 |
-0.33 |
| 18 |
Setzer, Sean T (13)
|
NCWOW |
25.40 |
25.48 |
0.08 |
| 20 |
Hyldahl, Cooper C (15)
|
NCWOW |
26.10 |
25.66 |
-0.44 |
| 21 |
Langtry, Ryan C (13)
|
NCWOW |
26.97 |
26.21 |
-0.76 |
| 22 |
Durham, William L (13)
|
NCWOW |
26.92 |
26.74 |
-0.18 |
| 23 |
King, Colvet J (16)
|
NCCFAC |
27.61 |
26.95 |
-0.66 |
| 24 |
Hill, Alexander T (14)
|
NCCFAC |
29.51 |
27.50 |
-2.01 |
| 25 |
Stuck, Evan J (13)
|
NCWOW |
28.09 |
28.00 |
-0.09 |
| 26 |
Eberhardt, Nathan L (14)
|
NCWOW |
29.81 |
29.33 |
-0.48 |
|
Gallagher, Jonathan T (16)
|
NCWOW |
27.48 |
NS |
- |
|
Kirby, Yates A (14)
|
NCWOW |
26.23 |
NS |
- |
|
Vitt, Carson A (14)
|
NCWOW |
30.03 |
NS |
- |
|
Snyder, Dakota S (15)
|
NCCFAC |
27.41 |
NS |
- |