| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Winchester, Austin O (16)
|
NCWOW |
53.90 |
52.56 |
-1.34 |
| 2 |
Settlemyre, Jeffrey P (17)
|
UNAT |
51.02 |
53.30 |
2.28 |
| 3 |
Balkus, Ethan J (17)
|
NCWOW |
54.19 |
55.01 |
0.82 |
| 4 |
Sterrett, William J (14)
|
NCWOW |
55.81 |
55.49 |
-0.32 |
| 5 |
Furbay, Patrick D (15)
|
NCCFAC |
54.63 |
55.53 |
0.90 |
| 6 |
Butner, Mark L (15)
|
NCWOW |
58.61 |
56.53 |
-2.08 |
| 7 |
Kirby, Evan N (16)
|
NCWOW |
55.78 |
58.04 |
2.26 |
| 8 |
Spencer, Brandon P (15)
|
NCCFAC |
58.95 |
59.03 |
0.08 |
| 9 |
Anselmo, Donald M (17)
|
NCBAC |
56.35 |
59.65 |
3.30 |
| 10 |
Luo, Matthew Z (15)
|
NCWOW |
1:02.10 |
1:00.39 |
-1.71 |
| 11 |
Kuhn, Lucas H (16)
|
NCBAC |
1:02.88 |
1:00.90 |
-1.98 |
| 12 |
Lyons, Frank E (16)
|
NCWOW |
1:08.22 |
1:04.01 |
-4.21 |
| 13 |
Gallek, Nicholas J (16)
|
NCWOW |
1:22.90 |
1:04.23 |
-18.67 |
| 14 |
Hill, Alexander T (14)
|
NCCFAC |
1:07.92 |
1:05.13 |
-2.79 |
| 15 |
Durham, William L (13)
|
NCWOW |
1:06.84 |
1:06.58 |
-0.26 |
| 16 |
Bell, Owen T (18)
|
NCBAC |
1:01.60 |
1:07.61 |
6.01 |
| 17 |
Brown, Philip M (13)
|
NCCFAC |
1:17.27 |
1:09.08 |
-8.19 |
| 18 |
Huo, Jack X (15)
|
NCCFAC |
1:13.80 |
1:09.12 |
-4.68 |
| 19 |
Gallagher, Jonathan T (16)
|
NCWOW |
1:11.04 |
1:09.15 |
-1.89 |
| 20 |
Nester, Nathaniel G (13)
|
NCBAC |
1:14.29 |
1:14.17 |
-0.12 |
| 21 |
Eberhardt, Nathan L (14)
|
NCWOW |
1:17.84 |
1:17.10 |
-0.74 |
|
Kirby, Yates A (14)
|
NCWOW |
1:04.93 |
NS |
- |