| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Girouard, Madelyn G (10)
|
NCRMY |
48.76 |
39.18 |
-9.58 |
| 1 |
Teague, Avalon G (9)
|
NCSTAR |
41.40 |
42.99 |
1.59 |
| 1 |
Johnson, Elyse C (9)
|
NCMOR |
49.05 |
47.72 |
-1.33 |
| 1 |
Vest, Sadie A (9)
|
NCSGSA |
|
54.71 |
- |
| 2 |
Schmitz, Madison G (9)
|
NCGFSC |
39.89 |
39.70 |
-0.19 |
| 2 |
Tse, Emma C (10)
|
NCMOR |
50.14 |
44.06 |
-6.08 |
| 2 |
Day, Lauren E (10)
|
NCHPSC |
57.27 |
48.87 |
-8.40 |
| 2 |
Carew, Rayleigh A (10)
|
NCGFSC |
1:01.11 |
54.94 |
-6.17 |
| 3 |
Brown, Makena R (10)
|
NCSGSA |
|
44.37 |
- |
| 3 |
Najera, Aileen F (10)
|
NCHPSC |
53.54 |
48.98 |
-4.56 |
| 3 |
Brugh, Anne S (10)
|
NCMOR |
50.20 |
55.51 |
5.31 |
| 4 |
Eggleston, Lucy A (10)
|
NCFAST |
52.92 |
44.49 |
-8.43 |
| 4 |
Becker, Megan L (9)
|
NCFAST |
58.52 |
50.45 |
-8.07 |
| 4 |
LaRue, Bailey A (9)
|
NCHPSC |
|
55.53 |
- |
| 5 |
Schmidt, Lorelei E (9)
|
NCMOR |
55.00 |
44.92 |
-10.08 |
| 5 |
Kaidel, Kaya R (10)
|
NCMOR |
50.00 |
50.49 |
0.49 |
| 5 |
Grove, Kaye T (10)
|
NCSGSA |
57.57 |
56.44 |
-1.13 |
| 6 |
Testa, Madeline C (9)
|
NCSGSA |
|
1:00.12 |
- |
| 6 |
Deans, Allyson C (10)
|
NCMOR |
55.00 |
45.98 |
-9.02 |
| 6 |
Lewis, Emily K (9)
|
NCSGSA |
53.11 |
51.73 |
-1.38 |
| 7 |
Ault, Serenity E (9)
|
NCSFSC |
|
1:00.52 |
- |
| 7 |
DiGiovanni, Francesca E (10)
|
NCMOR |
58.01 |
46.57 |
-11.44 |
| 7 |
Wolff, Ameilia N (10)
|
NCSFSC |
|
52.00 |
- |
| 8 |
McGuire, Molly S (10)
|
NCLOY |
1:01.62 |
1:02.38 |
0.76 |
| 8 |
McMasters, Sophie R (9)
|
NCSFSC |
43.47 |
46.58 |
3.11 |
| 8 |
Schilling, Abigail R (10)
|
NCSGSA |
52.74 |
52.64 |
-0.10 |
| 9 |
Bateson, Halena M (10)
|
NCHPSC |
|
1:11.74 |
- |
| 9 |
Buffum, Victoria R (9)
|
NCMOR |
50.00 |
46.60 |
-3.40 |
| 9 |
O'Donovan, Allison S (10)
|
NCMOR |
1:00.00 |
53.42 |
-6.58 |
|
Ciancanelli, Isabella R (9)
|
NCGFSC |
59.49 |
DQ |
- |
|
Thompson, Emma C (9)
|
NCMSA |
|
DQ |
- |
|
Ruelas Reyes, Samantha (10)
|
NCHPSC |
1:04.39 |
DQ |
- |
|
Ebert, Lia I (10)
|
NCMSA |
|
NS |
- |