| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schmitz, Madison G (9)
|
NCGFSC |
3:30.78 |
3:10.25 |
-20.53 |
| 1 |
Tse, Emma C (10)
|
NCMOR |
3:24.61 |
3:21.32 |
-3.29 |
| 1 |
Becker, Megan L (9)
|
NCFAST |
3:59.73 |
3:45.44 |
-14.29 |
| 2 |
Girouard, Madelyn G (10)
|
NCRMY |
3:56.83 |
3:16.12 |
-40.71 |
| 2 |
Wallace, Eliza A (10)
|
NCMSA |
3:47.30 |
3:22.92 |
-24.38 |
| 2 |
Cheung, Florence T (10)
|
NCGFSC |
3:56.11 |
3:45.75 |
-10.36 |
| 3 |
Johnson, Elyse C (9)
|
NCMOR |
3:44.99 |
3:35.13 |
-9.86 |
| 3 |
Day, Lauren E (10)
|
NCHPSC |
4:19.18 |
3:48.46 |
-30.72 |
| 4 |
Teague, Avalon G (9)
|
NCSTAR |
3:30.36 |
3:38.74 |
8.38 |
| 4 |
DiGiovanni, Francesca E (10)
|
NCMOR |
3:46.19 |
3:50.50 |
4.31 |
| 5 |
Eggleston, Lucy A (10)
|
NCFAST |
3:48.73 |
3:40.09 |
-8.64 |
| 6 |
Najera, Aileen F (10)
|
NCHPSC |
4:07.48 |
3:40.75 |
-26.73 |
|
Ruelas Reyes, Samantha (10)
|
NCHPSC |
|
DQ |
- |