| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pena, Victoria (12)
|
FGFKSC |
35.66 |
34.34 |
-1.32 |
| 2 |
Gelin, Mia M (12)
|
FGEA |
35.01 |
35.03 |
0.02 |
| 3 |
Mercado, Keira R (11)
|
FGEA |
36.95 |
35.99 |
-0.96 |
| 4 |
Acevedo, Saravalentina (11)
|
FGCM |
35.98 |
36.15 |
0.17 |
| 5 |
Garrocho, Marffa (12)
|
FGMBS |
45.91 |
37.57 |
-8.34 |
| 6 |
Ryzhova, Anna (12)
|
FGMSAT |
|
39.48 |
- |
| 7 |
Lowell, Chrislyn M (12)
|
FGFKSC |
44.80 |
39.52 |
-5.28 |
| 8 |
Halliburton, Mercedes (11)
|
FGCANE |
41.99 |
40.42 |
-1.57 |
| 9 |
Robbins, Laney (11)
|
FGFKSC |
44.57 |
41.15 |
-3.42 |
| 10 |
Kuraeva, Ekaterina (12)
|
FGMSAT |
|
41.17 |
- |
| 11 |
Suarez Alarcon, Gabriella A (11)
|
FGMBS |
41.38 |
41.31 |
-0.07 |
| 12 |
Mangel, Hailey (11)
|
FGFKSC |
|
44.98 |
- |
| 13 |
Guerra, Heidi (11)
|
FGFKSC |
|
45.31 |
- |
| 14 |
Volovich, Elizabeth (11)
|
FGSOFL |
48.52 |
45.46 |
-3.06 |
| 15 |
Sorokina, Daria (11)
|
FGMSAT |
|
45.79 |
- |
| 16 |
Scheingoltz, Lillian (11)
|
FGMCDA |
55.89 |
50.30 |
-5.59 |
|
Burnham-Ugalde, Alexandra M (11)
|
FGMBS |
46.57 |
DQ |
- |