| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark, McKenna (15)
|
ONGCAC |
5:00.55 |
4:54.96 |
-5.59 |
| 2 |
Kisker, Elizabeth M (17)
|
NITWST |
5:06.49 |
5:11.04 |
4.55 |
| 3 |
Carrig, Marissa K (18)
|
NITWST |
5:16.93 |
5:12.77 |
-4.16 |
| 4 |
Smith, Paige (16)
|
NIAMTS |
5:05.31 |
5:13.08 |
7.77 |
| 5 |
Kidder, Elizabeth A (15)
|
NIHAST |
5:02.53 |
5:14.57 |
12.04 |
| 6 |
Webster, Emma E (16)
|
NIUN |
5:33.13 |
5:16.58 |
-16.55 |
| 7 |
Pasquarella, Paige E (18)
|
NITWST |
5:17.09 |
5:17.11 |
0.02 |
| 8 |
Filipski, Julie A (15)
|
NITWST |
|
5:17.34 |
- |
| 9 |
Bargnesi, Veronica M (15)
|
NITTSC |
5:26.51 |
5:17.35 |
-9.16 |
| 10 |
Granger, Isabel C (15)
|
NIMERC |
5:07.04 |
5:19.64 |
12.60 |
| 11 |
Strongitharm, Madelyn (15)
|
ONGCAC |
6:00.00 |
5:25.90 |
-34.10 |
| 12 |
Mete, Olivia (16)
|
ONGCAC |
6:00.00 |
5:26.96 |
-33.04 |
| 13 |
Herman, Olivia T (15)
|
NITWST |
|
5:32.42 |
- |
| 14 |
Dimmig, Liliana L (17)
|
NITTSC |
|
5:36.09 |
- |
| 15 |
Scoma, Adriana C (15)
|
NIBAAC |
5:40.52 |
5:37.37 |
-3.15 |
| 16 |
Koschuk, Olivia J (17)
|
NITTSC |
5:55.75 |
5:41.72 |
-14.03 |
|
Hetrick, Paige M (16)
|
NIUN |
4:21.57 |
NS |
- |