| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Amering, Sophie M (13)
|
NIHAST |
31.94 |
29.99 |
-1.95 |
| 2 |
Harris, Saiya'Kaede Z (13)
|
NIRRST |
31.48 |
30.91 |
-0.57 |
| 3 |
Tytler, Candace L (13)
|
NIIRON |
31.30 |
31.02 |
-0.28 |
| 4 |
Galusha, Alexis P (13)
|
NIGRSC |
31.67 |
31.09 |
-0.58 |
| 5 |
Utz, Natalie R (13)
|
NIGCOM |
31.93 |
31.82 |
-0.11 |
| 6 |
Werner, Riley K (13)
|
NIVICT |
33.21 |
32.32 |
-0.89 |
| 7 |
Uchal, Hannah L (13)
|
NIWEBS |
31.58 |
32.49 |
0.91 |
| 8 |
Cassidy, Alexa E (13)
|
NISDSC |
33.71 |
33.53 |
-0.18 |
| 9 |
Wideman, Madeline J (13)
|
NIWEBS |
32.37 |
34.26 |
1.89 |
| 10 |
Haak, Ariel E (13)
|
NIRRST |
36.62 |
35.19 |
-1.43 |
|
Stepanek, Abbigail L (13)
|
NIHAST |
32.08 |
NS |
- |
|
Selioutski, Leeva A (13)
|
NISDSC |
35.97 |
NS |
- |