| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carey, Emilia M (9)
|
NIIRON |
50.25 |
43.73 |
-6.52 |
| 2 |
Ramsperger, Lily A (7)
|
NISDSC |
53.05 |
46.86 |
-6.19 |
| 3 |
Boggio, Addison L (8)
|
NIQUIK |
53.75 |
47.15 |
-6.60 |
| 4 |
Miller, Amanda C (9)
|
NIVICT |
50.42 |
47.88 |
-2.54 |
| 5 |
Wagner, Ryleigh J (7)
|
NISDSC |
48.13 |
47.89 |
-0.24 |
| 6 |
Crowley, Lila M (9)
|
NIGRSC |
48.90 |
48.26 |
-0.64 |
| 7 |
Corcoran, Riley A (9)
|
NISDSC |
53.52 |
50.17 |
-3.35 |
| 8 |
Galley, Morgan C (9)
|
NIRRST |
1:02.75 |
51.28 |
-11.47 |
| 9 |
Chen, Sophie Y (8)
|
NISDSC |
50.86 |
51.30 |
0.44 |
| 10 |
Garigen, Natalie K (8)
|
NIWEBS |
50.52 |
51.42 |
0.90 |
| 11 |
Weber, Sophia R (7)
|
NIWEBS |
52.94 |
51.57 |
-1.37 |
| 12 |
Van Hout, Kennyn A (8)
|
NISDSC |
54.57 |
53.36 |
-1.21 |
| 13 |
Quinto, Lilian M (8)
|
NIIRON |
54.09 |
53.59 |
-0.50 |
| 14 |
Zebrak, Abigail R (7)
|
NISDSC |
|
53.61 |
- |
| 15 |
Corcoran, Alexa G (7)
|
NISDSC |
59.71 |
53.71 |
-6.00 |
| 16 |
Coon, Chloe A (8)
|
NIGCOM |
1:00.54 |
53.90 |
-6.64 |
| 17 |
Baum, Scarlett A (9)
|
NIGRSC |
57.36 |
54.37 |
-2.99 |
| 18 |
Black, Lily A (8)
|
NISDSC |
|
55.29 |
- |
| 19 |
Utz, Naomi L (7)
|
NIGCOM |
|
56.96 |
- |
| 20 |
Zebrak, Rebecca S (7)
|
NISDSC |
|
57.01 |
- |
| 21 |
Balkman, Zuri A (8)
|
NIGRSC |
|
57.70 |
- |
| 22 |
Maaraba, Hanna M (8)
|
NIPACK |
59.88 |
58.77 |
-1.11 |
| 23 |
Gonzalez, Alexis W (9)
|
NIPACK |
55.45 |
59.19 |
3.74 |
| 24 |
Ciminelli, Sarah P (8)
|
NIGRSC |
|
59.58 |
- |
| 25 |
Ayers, Brianna S (8)
|
NIGRSC |
1:04.16 |
1:03.73 |
-0.43 |
| 26 |
Lamphere, Carolyn L (8)
|
NIGCOM |
1:01.83 |
1:08.44 |
6.61 |
| 27 |
Castellano, Sophia M (6)
|
NIGCOM |
1:13.32 |
1:08.83 |
-4.49 |
| 28 |
Hendy, Caroline L (7)
|
NIGCOM |
1:08.28 |
1:15.20 |
6.92 |
| 29 |
Hartnett, Olivia G (7)
|
NIGCOM |
|
1:22.35 |
- |
|
Duran, Heidi K (9)
|
NIGRSC |
50.91 |
NS |
- |
|
Thaney, Brigid G (9)
|
NIGRSC |
54.69 |
NS |
- |
|
Weber, Mackenzie N (5)
|
NIGRSC |
|
NS |
- |
|
McFadden Olivier, Camryn (8)
|
NIPACK |
|
NS |
- |