| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Geary, Kaitlyn J (11)
|
NIMERC |
39.34 |
34.71 |
-4.63 |
| 2 |
Bouwens, Emma L (11)
|
NIHAST |
38.45 |
36.67 |
-1.78 |
| 3 |
Delehanty, Maeve K (11)
|
NIRRST |
38.00 |
36.93 |
-1.07 |
| 4 |
Share, Elizabeth A (11)
|
NIPMSC |
38.86 |
37.41 |
-1.45 |
| 5 |
Robinson, Sara C (11)
|
NISDSC |
47.02 |
38.55 |
-8.47 |
| 6 |
Taylor, Janey A (11)
|
NIVICT |
41.35 |
38.75 |
-2.60 |
| 7 |
Smolinski, Evelynn J (11)
|
NIHAST |
42.78 |
39.73 |
-3.05 |
| 8 |
Beutner, Ella R (11)
|
NIQUIK |
46.36 |
41.07 |
-5.29 |
| 9 |
Greisch, Alisha D (11)
|
NIGRSC |
42.29 |
41.47 |
-0.82 |
| 10 |
Resch, Rebecka J (11)
|
NIGRSC |
42.34 |
42.95 |
0.61 |
| 11 |
Kuhlkin, Elizabeth C (11)
|
NIGCOM |
43.71 |
43.21 |
-0.50 |
| 12 |
Rist, Olivia B (11)
|
NIHAST |
53.09 |
47.03 |
-6.06 |
| 13 |
Farina, Samantha R (11)
|
NIGRSC |
48.81 |
49.53 |
0.72 |
| 14 |
Thurston, Lauren A (11)
|
NIGCOM |
49.88 |
50.19 |
0.31 |
| 15 |
Na, Semyeong B (11)
|
NIGCOM |
|
54.28 |
- |