| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kazmark, Raelyn J (13)
|
NIVICT |
2:44.89 |
2:44.02 |
-0.87 |
| 2 |
Amering, Sophie M (13)
|
NIHAST |
3:35.96 |
2:53.99 |
-41.97 |
| 3 |
Doughty, Emma C (13)
|
NISDSC |
2:57.26 |
2:59.58 |
2.32 |
| 4 |
Werner, Riley K (13)
|
NIVICT |
3:08.89 |
3:00.85 |
-8.04 |
| 5 |
Heckle, Madelyn E (13)
|
NIGRSC |
|
3:05.67 |
- |
| 6 |
Galusha, Alexis P (13)
|
NIGRSC |
3:12.96 |
3:12.00 |
-0.96 |
| 7 |
Harris, Saiya'Kaede Z (13)
|
NIRRST |
3:10.78 |
3:14.91 |
4.13 |
| 8 |
Wideman, Madeline J (13)
|
NIWEBS |
4:10.00 |
3:21.39 |
-48.61 |
|
Stepanek, Abbigail L (13)
|
NIHAST |
3:02.96 |
NS |
- |
|
Rees, Hannah E (13)
|
NIGRSC |
2:50.68 |
NS |
- |
|
McGraw, Caroline (13)
|
NIRRST |
|
NS |
- |