| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (10)
|
NIVICT |
2:33.60 |
2:32.60 |
-1.00 |
| 2 |
Wamsley, Sarah E (10)
|
NIVELO |
2:56.54 |
2:47.48 |
-9.06 |
| 3 |
Sahin, Hazal (10)
|
NIPACK |
2:44.67 |
2:48.41 |
3.74 |
| 4 |
Kunze, Sarah A (9)
|
NIPACK |
2:50.10 |
2:49.48 |
-0.62 |
| 5 |
Reilly, Bridget F (10)
|
NISTAR |
3:01.13 |
2:51.74 |
-9.39 |
| 6 |
Zagrobelny, Erin R (10)
|
NIEAST |
2:56.96 |
2:52.63 |
-4.33 |
| 7 |
Cywinski, Anna C (10)
|
NIVICT |
2:50.95 |
2:52.94 |
1.99 |
| 8 |
Crossing, Natalie E (10)
|
NIVICT |
2:59.74 |
2:56.31 |
-3.43 |
| 9 |
Anderson, Madden (10)
|
NIVELO |
3:00.57 |
2:56.94 |
-3.63 |
| 10 |
Boyer, Chloe G (9)
|
NIISWM |
3:03.01 |
2:58.48 |
-4.53 |
| 11 |
Geary, Maria E (9)
|
NIMERC |
3:01.94 |
2:58.88 |
-3.06 |
| 12 |
Tytler, Elizabeth (10)
|
NIIRON |
3:01.56 |
2:58.95 |
-2.61 |
| 13 |
Walier, Natalie K (10)
|
NITWST |
3:06.09 |
3:01.80 |
-4.29 |
| 14 |
Schwandt, Natalie K (10)
|
NIGRAT |
3:02.77 |
3:02.27 |
-0.50 |
| 15 |
Upcraft, Malia L (10)
|
NIMETS |
3:04.08 |
3:03.42 |
-0.66 |
| 16 |
Manciocchi, Kayden M (10)
|
NIHAST |
3:02.50 |
3:03.69 |
1.19 |
| 17 |
Choroser, Penelope T (10)
|
NITWST |
3:04.18 |
3:05.29 |
1.11 |
| 18 |
Kleinmann, Isabelle K (10)
|
NIHAMB |
3:07.32 |
3:05.37 |
-1.95 |