| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
BLAKE, HANNAH G (10)
|
NISWAT |
|
50.92 |
- |
| 2 |
COOK, AMELIA A (10)
|
NIHAMB |
53.42 |
51.56 |
-1.86 |
| 3 |
HORN, ERIN E (10)
|
NIEAST |
53.68 |
51.84 |
-1.84 |
| 4 |
ILLIG, MCKENNA R (10)
|
NISWAT |
55.53 |
54.07 |
-1.46 |
| 5 |
BARRERA-HAGNER, ANANYA S (10)
|
NIEAST |
53.45 |
55.21 |
1.76 |
| 6 |
KERR, BELL L (10)
|
NIPVSC |
52.36 |
56.87 |
4.51 |
| 7 |
CASEY, TAYLOR R (10)
|
NISWAT |
1:01.53 |
1:01.78 |
0.25 |
| 8 |
SCHULTZ, KENDRA M (10)
|
NIPVSC |
1:01.76 |
1:06.45 |
4.69 |
|
KRAJEWSKI, DANIELLE L (10)
|
NIGWST |
|
DQ |
- |
|
MCGILL, ROSE M (10)
|
NIHAMB |
1:01.67 |
DQ |
- |
|
YASUREK, JULIANNA J (10)
|
NIPVSC |
2:02.40 |
DQ |
- |