| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
El Ghissassi, Sidra S (8)
|
NIVEST |
39.45 |
36.15 |
-3.30 |
| 2 |
Wang, Bethany B (6)
|
NIKRKN |
38.91 |
37.41 |
-1.50 |
| 3 |
Robinson, Elizabeth K (9)
|
NIWAAC |
43.85 |
40.95 |
-2.90 |
| 4 |
Schrader, Olivia Q (9)
|
NIUNAC |
43.43 |
41.96 |
-1.47 |
| 5 |
Kondrup, Kenzington A (9)
|
NIUNAC |
48.21 |
42.34 |
-5.87 |
| 6 |
Leonard, Kaitllyn S (8)
|
NIWAAC |
44.26 |
42.52 |
-1.74 |
| 7 |
Leahey, Emme A (8)
|
NIUNAC |
46.15 |
43.68 |
-2.47 |
| 8 |
Denlinger, Elizabeth J (9)
|
NIWAAC |
42.64 |
43.81 |
1.17 |
| 9 |
Stank, Amelia D (6)
|
NIUNAC |
52.74 |
46.39 |
-6.35 |
| 10 |
Hall, Liliana E (9)
|
NIKRKN |
52.15 |
47.09 |
-5.06 |
| 11 |
Knowlton, Ava L (9)
|
NIKRKN |
51.44 |
48.58 |
-2.86 |
| 12 |
Henderson, Madeline R (9)
|
NIWAAC |
51.38 |
49.16 |
-2.22 |
| 13 |
Cox, Hannah A (7)
|
NIHAWK |
|
50.82 |
- |
| 14 |
Spencer, Danielle H (6)
|
NIWAAC |
1:05.83 |
59.16 |
-6.67 |
| 15 |
Mahon, Lauren H (7)
|
NIHAWK |
|
1:00.27 |
- |
| 16 |
Long, Kathryn M (8)
|
NIKRKN |
|
1:03.51 |
- |
| 17 |
Homer, Olivia K (7)
|
NIWAAC |
1:05.25 |
1:04.29 |
-0.96 |
|
Hunter, Isabel G (9)
|
NIUNAC |
51.37 |
NS |
- |
|
Chesna, Natalie C (9)
|
NIUNAC |
48.66 |
NS |
- |
|
Welles, Lauryn E (8)
|
NIWAAC |
|
NS |
- |