| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kuksenkova, Maria M (9)
|
NIKRKN |
45.34 |
43.32 |
-2.02 |
| 2 |
El Ghissassi, Sidra S (8)
|
NIVEST |
48.39 |
45.52 |
-2.87 |
| 3 |
Blakely-Armitage, Fiona S (9)
|
NIISWM |
57.13 |
46.67 |
-10.46 |
| 4 |
Leonard, Kaitllyn S (8)
|
NIWAAC |
49.99 |
47.29 |
-2.70 |
| 5 |
Schrader, Olivia Q (9)
|
NIUNAC |
59.99 |
47.61 |
-12.38 |
| 6 |
Ryan, Natalie C (9)
|
NIUNAC |
50.82 |
49.14 |
-1.68 |
| 7 |
Denlinger, Elizabeth J (9)
|
NIWAAC |
48.14 |
50.55 |
2.41 |
| 8 |
Henderson, Madeline R (9)
|
NIWAAC |
57.40 |
54.51 |
-2.89 |
| 9 |
Hennessey, Haley E (9)
|
NIUNAC |
55.00 |
54.92 |
-0.08 |
| 10 |
Knowlton, Ava L (9)
|
NIKRKN |
59.16 |
56.88 |
-2.28 |
| 11 |
Homer, Olivia K (7)
|
NIWAAC |
|
57.32 |
- |
| 12 |
Long, Kathryn M (8)
|
NIKRKN |
|
59.00 |
- |
| 13 |
Chin, Evelyn (7)
|
NIKRKN |
|
59.24 |
- |
| 14 |
Yu, Vicky Z (6)
|
NIKRKN |
|
1:03.87 |
- |
| 15 |
Spencer, Danielle H (6)
|
NIWAAC |
1:18.91 |
1:10.18 |
-8.73 |
| 16 |
Mahon, Lauren H (7)
|
NIHAWK |
|
1:14.47 |
- |
|
Welles, Lauryn E (8)
|
NIWAAC |
|
NS |
- |