| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chang, Sydney X (6)
|
PVRMSC |
|
24.40 |
- |
| 2 |
Groves, Charlotte (6)
|
PVASA |
24.48 |
24.61 |
0.13 |
| 3 |
Cassidy, Peyton (6)
|
PVRMSC |
24.50 |
24.83 |
0.33 |
| 4 |
Mejia-Mardeeva, Annabel M (6)
|
PVRMSC |
31.41 |
25.73 |
-5.68 |
| 5 |
Kim, Charlotte Y (6)
|
PVRMSC |
31.07 |
25.94 |
-5.13 |
| 6 |
Zettler, Grace A (6)
|
PVASA |
|
27.12 |
- |
| 7 |
Hefferon, Louise A (6)
|
PVASA |
|
27.22 |
- |
| 8 |
Averill, Karina K (6)
|
PVRMSC |
26.30 |
27.63 |
1.33 |
| 9 |
Gredder, Lauren (6)
|
PVRMSC |
28.19 |
30.46 |
2.27 |
| 10 |
Chen, Zella (6)
|
PVRMSC |
27.96 |
31.40 |
3.44 |
| 11 |
Santos, Alaina (6)
|
PVRMSC |
30.35 |
31.70 |
1.35 |
| 12 |
Wu, Judy (6)
|
PVRMSC |
32.00 |
31.97 |
-0.03 |
| 13 |
Ferullo, Blake (6)
|
PVRMSC |
29.09 |
32.67 |
3.58 |
| 14 |
Wang, Ivy Y (6)
|
PVRMSC |
30.40 |
33.59 |
3.19 |
| 15 |
Netram, Serena R (6)
|
PVRMSC |
30.62 |
34.50 |
3.88 |
| 16 |
He, Athena C (6)
|
PVRMSC |
|
34.95 |
- |
| 17 |
Kwon, Eleanor (6)
|
PVRMSC |
28.12 |
35.26 |
7.14 |
| 18 |
Heitzmann, Caitlyn (6)
|
PVRMSC |
32.72 |
36.39 |
3.67 |
| 19 |
Walelign, Mahelet (6)
|
PVRMSC |
32.22 |
38.10 |
5.88 |
| 20 |
Liang, Bo-Ya (6)
|
PVRMSC |
32.68 |
41.07 |
8.39 |
| 21 |
Bell, Aria G (6)
|
PVRMSC |
36.47 |
41.76 |
5.29 |
| 22 |
Trepkowski, Sabrina N (5)
|
PVRMSC |
42.59 |
41.88 |
-0.71 |
|
Lee, Kaitlin (6)
|
PVRMSC |
27.79 |
NS |
- |
|
Hu, Sophia G (6)
|
PVMSSC |
40.91 |
NS |
- |