| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gussom, Rajah L (15)
|
PVNCAP |
27.68 |
27.64 |
-0.04 |
| 2 |
Yard, Janai N (16)
|
PVNCAP |
28.38 |
28.34 |
-0.04 |
| 3 |
Stovall, Zora L (15)
|
PVNCAP |
26.25 |
28.39 |
2.14 |
| 4 |
Rosebure, Tai A (17)
|
PVNCAP |
28.42 |
29.67 |
1.25 |
| 5 |
Hall, Zohra S (15)
|
PVNCAP |
28.98 |
30.11 |
1.13 |
| 6 |
Chamorro-Beckenhauer, Irene (17)
|
PVDCPR |
30.08 |
30.29 |
0.21 |
| 7 |
Smith, Zoli (15)
|
PVDCPR |
30.73 |
31.67 |
0.94 |
| 8 |
Russell, Dhana K (16)
|
PVPGPR |
30.86 |
32.86 |
2.00 |
| 9 |
Sanabria, Sabrina (17)
|
PVDCPR |
31.57 |
33.08 |
1.51 |
| 10 |
Akil, Imani N (15)
|
PVPGPR |
29.43 |
40.38 |
10.95 |
|
Willoughby, Wrayzene L (17)
|
PVDCPR |
28.38 |
NS |
- |
|
Waters, Avianna B (15)
|
PVPGPR |
36.29 |
NS |
- |