| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stovall, Langston A (13)
|
PVNCAP |
25.95 |
25.43 |
-0.52 |
| 2 |
Wood, Diallo N (14)
|
PVDCPR |
25.50 |
25.57 |
0.07 |
| 3 |
Anders, Eric R (13)
|
PVNCAP |
26.31 |
26.18 |
-0.13 |
| 4 |
Robinson, Parker (14)
|
PVDCPR |
25.97 |
26.23 |
0.26 |
| 5 |
Byrd, Khaleb A (13)
|
PVNCAP |
26.60 |
27.10 |
0.50 |
| 6 |
Toppin, Jamari E (14)
|
PVDCPR |
26.92 |
27.42 |
0.50 |
| 7 |
Byrne-Kvalsvik, Henry N (14)
|
PVDCPR |
28.61 |
28.58 |
-0.03 |
| 8 |
Lam, Mateo F (14)
|
PVDCPR |
29.63 |
28.79 |
-0.84 |
| 9 |
McLean, Markale T (13)
|
PVPGPR |
29.17 |
30.25 |
1.08 |
| 10 |
Kiley, Aidan (14)
|
PVDCPR |
31.07 |
31.92 |
0.85 |
| 11 |
Young, Ceyon M (13)
|
PVNCAP |
29.13 |
32.16 |
3.03 |
| 12 |
Simpson, Javier D (13)
|
PVDCPR |
32.81 |
34.28 |
1.47 |
| 13 |
Saunders, Kai-Rahn A (13)
|
PVPGPR |
|
46.35 |
- |
|
George, Akinola T (14)
|
PVDCPR |
31.99 |
NS |
- |