| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mattis, Brianna A (12)
|
PVNCAP |
36.22 |
36.20 |
-0.02 |
| 2 |
Custis, Rayven M (12)
|
PVUN-D |
37.54 |
36.95 |
-0.59 |
| 3 |
Watson, Karah E (12)
|
PVNCAP |
39.90 |
39.68 |
-0.22 |
| 4 |
Nowak, Jessica G (11)
|
PVDCPR |
38.44 |
40.08 |
1.64 |
| 5 |
Callahan, Katharine P (12)
|
PVDCPR |
|
40.47 |
- |
| 6 |
Brown-Andrews, Zora I (11)
|
PVDCPR |
46.30 |
44.28 |
-2.02 |
| 7 |
Clark, Makenna (11)
|
PVDCPR |
46.23 |
46.42 |
0.19 |
| 8 |
Morton, Lily Grace M (12)
|
PVDCPR |
50.54 |
47.02 |
-3.52 |
| 9 |
Brown, Gabrielle A (11)
|
PVPGPR |
44.40 |
47.48 |
3.08 |
| 10 |
Hale, Laila P (12)
|
PVPGPR |
49.08 |
53.56 |
4.48 |
| 11 |
McClain, Kennedy B (12)
|
PVPGPR |
50.20 |
54.88 |
4.68 |
| 12 |
Atwood, Kadence R (11)
|
PVDCPR |
58.25 |
57.22 |
-1.03 |
| 13 |
Diallo, Genaba H (12)
|
PVDCPR |
|
57.72 |
- |
|
Behrens, Julie M (12)
|
PVDCPR |
1:12.45 |
DQ |
- |
|
Porter, Sarah P (12)
|
PVDCPR |
|
NS |
- |