| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Firestone, Sydney A (10)
|
PVPM |
36.84 |
37.48 |
0.64 |
| 2 |
Porter, Drew A (10)
|
PVMACH |
37.16 |
37.69 |
0.53 |
| 3 |
Kritsky, Josephina (9)
|
PVSNOW |
39.89 |
38.44 |
-1.45 |
| 4 |
Ismail, Mariam O (10)
|
PVSNOW |
43.89 |
38.47 |
-5.42 |
| 5 |
Holmes, Tara M (9)
|
PVYORK |
39.65 |
39.36 |
-0.29 |
| 6 |
Maitland, Sydney L (10)
|
PVMACH |
39.00 |
39.57 |
0.57 |
| 6 |
Nguyen, Lien J (9)
|
PVPM |
39.51 |
39.57 |
0.06 |
| 8 |
Thaivalappil, Maya E (9)
|
PVPM |
|
40.39 |
- |
| 9 |
Nagle, Audrey R (10)
|
PVMACH |
44.44 |
42.84 |
-1.60 |
| 10 |
Bordovsky, Abigail L (10)
|
PVUN-M |
42.32 |
43.22 |
0.90 |
| 11 |
Strickland, Virginia B (9)
|
PVPM |
|
44.46 |
- |
| 12 |
Christofferson, Marie N (8)
|
PVMACH |
|
44.73 |
- |
| 13 |
Sparrenberger, Riley M (8)
|
PVSNOW |
43.00 |
45.70 |
2.70 |
| 14 |
Lahr, Leah (8)
|
PVPM |
47.09 |
47.70 |
0.61 |
| 15 |
Li, Samantha W (9)
|
PVMACH |
|
51.30 |
- |
|
Masten, Payton E (9)
|
PVSNOW |
36.01 |
NS |
- |