| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fredericks, Sophie C (10)
|
PVMACH |
43.60 |
45.07 |
1.47 |
| 2 |
Thaivalappil, Maya E (9)
|
PVPM |
|
50.65 |
- |
| 3 |
Williams, Jill C (9)
|
PVMACH |
53.77 |
51.30 |
-2.47 |
| 4 |
Holmes, Tara M (9)
|
PVYORK |
53.35 |
53.91 |
0.56 |
| 5 |
Bordovsky, Abigail L (10)
|
PVUN-M |
53.71 |
54.21 |
0.50 |
| 6 |
Maitland, Sydney L (10)
|
PVMACH |
55.06 |
54.34 |
-0.72 |
| 7 |
Nagle, Audrey R (10)
|
PVMACH |
59.77 |
55.51 |
-4.26 |
| 8 |
Porter, Drew A (10)
|
PVMACH |
53.83 |
55.96 |
2.13 |
| 9 |
Kritsky, Josephina (9)
|
PVSNOW |
56.89 |
58.74 |
1.85 |
| 10 |
Lahr, Leah (8)
|
PVPM |
1:03.09 |
59.40 |
-3.69 |
| 11 |
Strickland, Virginia B (9)
|
PVPM |
|
59.60 |
- |
| 12 |
Li, Samantha W (9)
|
PVMACH |
1:06.54 |
1:01.09 |
-5.45 |
| 13 |
Christofferson, Marie N (8)
|
PVMACH |
1:03.48 |
1:06.28 |
2.80 |
|
Masten, Payton E (9)
|
PVSNOW |
46.78 |
NS |
- |