| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blake, Madeline C (12)
|
PVFXFX |
39.53 |
36.07 |
-3.46 |
| 2 |
Campbell, Elaine N (12)
|
PVMAKO |
34.63 |
36.11 |
1.48 |
| 3 |
Eziemefe, Adaora I (12)
|
PVMACH |
37.86 |
36.19 |
-1.67 |
| 4 |
Sutherland, Lauren E (12)
|
PVPM |
|
36.76 |
- |
| 5 |
Schloth, Katherine I (11)
|
PVPM |
40.37 |
37.47 |
-2.90 |
| 6 |
Lahr, Alyson G (11)
|
PVPM |
37.08 |
37.58 |
0.50 |
| 7 |
Sanchez, Erin V (12)
|
PVMACH |
|
39.14 |
- |
| 8 |
Altenburg, Abigail W (12)
|
PVPM |
40.46 |
39.83 |
-0.63 |
| 9 |
Sanchez, Lily A (12)
|
PVYORK |
40.19 |
40.45 |
0.26 |
| 10 |
Golembeski, Bailey C (11)
|
PVMACH |
42.21 |
40.76 |
-1.45 |
| 11 |
Sharrer, Katherine C (12)
|
PVYORK |
42.40 |
40.94 |
-1.46 |
| 12 |
Kim, Allison S (11)
|
PVMAC |
43.42 |
42.04 |
-1.38 |
| 13 |
Sturgill, Lydia K (11)
|
PVMACH |
43.38 |
42.75 |
-0.63 |
| 14 |
Crapco-Wang, Caitlyn L (11)
|
PVMACH |
46.45 |
44.86 |
-1.59 |
|
Tourbaf, Eliza J (12)
|
PVMACH |
36.05 |
NS |
- |
|
Dixon, Charlotte J (12)
|
PVYORK |
34.29 |
NS |
- |
|
Fleming, Sharli A (11)
|
PVMACH |
39.46 |
NS |
- |