| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fredericks, Sophie C (10)
|
PVMACH |
38.36 |
40.70 |
2.34 |
| 2 |
McAllister, Maia C (10)
|
PVPM |
45.09 |
43.76 |
-1.33 |
| 3 |
Firestone, Sydney A (10)
|
PVPM |
41.29 |
43.83 |
2.54 |
| 4 |
Holmes, Tara M (9)
|
PVUN |
47.58 |
44.06 |
-3.52 |
| 5 |
Hamilton, Alexandria I (10)
|
PVAAC |
44.04 |
44.55 |
0.51 |
| 6 |
Strickland, Virginia B (9)
|
PVPM |
|
46.71 |
- |
| 7 |
Nagle, Audrey R (10)
|
PVUN-M |
50.02 |
47.34 |
-2.68 |
| 8 |
Porter, Drew A (10)
|
PVMACH |
47.09 |
50.20 |
3.11 |
| 9 |
Park, Gianna S (9)
|
PVMAC |
54.35 |
50.84 |
-3.51 |
| 10 |
Lahr, Leah (8)
|
PVPM |
50.09 |
51.04 |
0.95 |
| 11 |
Nguyen, Lien J (9)
|
PVPM |
48.18 |
51.12 |
2.94 |
| 12 |
Christofferson, Marie N (8)
|
PVMACH |
53.10 |
58.52 |
5.42 |
|
Thaivalappil, Maya E (9)
|
PVPM |
45.09 |
NS |
- |