| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Campbell, Sydney K (7)
|
PVMSSC |
20.21 |
18.16 |
-2.05 |
| 2 |
Blaha, Sienna K (7)
|
MDMSC |
23.04 |
22.02 |
-1.02 |
| 3 |
Bascope, Maya M (7)
|
PVMSSC |
26.62 |
24.29 |
-2.33 |
| 4 |
Batty, Leah M (7)
|
PVPAC |
26.95 |
24.95 |
-2.00 |
| 5 |
Keating, Hannah M (7)
|
PVTIBU |
29.09 |
26.48 |
-2.61 |
| 6 |
Bice, Annabella Y (7)
|
PVPAC |
29.44 |
27.49 |
-1.95 |
| 7 |
Minalu, Kalkidan A (7)
|
PVDCPR |
29.42 |
29.13 |
-0.29 |
| 8 |
Cinquegrane, Marie (7)
|
PVWEA |
|
30.13 |
- |
| 9 |
Bayona, Laura A (7)
|
PVMSSC |
38.96 |
31.92 |
-7.04 |
| 10 |
Hurd, Rileigh R (7)
|
PVFAST |
|
32.65 |
- |
| 11 |
Jones-Puthoff, Daniella L (7)
|
PVFAST |
33.77 |
32.79 |
-0.98 |
| 12 |
Biggs, Angelina O (7)
|
PVTIBU |
|
38.27 |
- |
|
Gramiccioni, Ila V (7)
|
PVPAC |
30.88 |
DQ |
- |
|
Alexander-Fettus, Jane M (7)
|
PVDCPR |
32.90 |
NS |
- |