| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaston, Aleah S (7)
|
PVNCAP |
49.73 |
46.48 |
-3.25 |
| 2 |
Feng, Sophie F (7)
|
PVNCAP |
52.82 |
48.15 |
-4.67 |
| 3 |
Harms, Stella J (6)
|
PVNCAP |
59.76 |
49.05 |
-10.71 |
| 4 |
Naum, Alexandra S (7)
|
PVNCAP |
58.56 |
50.21 |
-8.35 |
| 5 |
Polifko, Louise C (7)
|
PVNCAP |
55.00 |
50.37 |
-4.63 |
| 6 |
Klein, Carolina A (7)
|
PVNCAP |
|
51.87 |
- |
| 7 |
Muller, Quinn L (7)
|
PVNCAP |
58.33 |
52.14 |
-6.19 |
| 8 |
Orgad, Abigail S (7)
|
PVNCAP |
|
52.34 |
- |
| 9 |
Buchanan, Brooke Taylor (7)
|
PVNCAP |
53.57 |
53.27 |
-0.30 |
| 10 |
Eisenhart, Abigail H (7)
|
PVNCAP |
50.02 |
55.40 |
5.38 |
| 11 |
Benevento, Ava K (7)
|
PVNCAP |
54.17 |
55.83 |
1.66 |
| 12 |
Walker, Beatrice E (7)
|
PVNCAP |
|
59.24 |
- |
| 13 |
Shive, Keira X (6)
|
PVNCAP |
1:16.14 |
59.66 |
-16.48 |
| 14 |
Thompson, Addison M (7)
|
PVNCAP |
56.17 |
1:01.26 |
5.09 |
| 15 |
Fogle, Madison N (7)
|
PVNCAP |
|
1:04.40 |
- |
| 16 |
Lucchese, Alexandra G (7)
|
PVNCAP |
|
1:11.14 |
- |
| 17 |
Patel, Meera A (6)
|
PVNCAP |
1:26.94 |
1:16.99 |
-9.95 |
| 18 |
Hennelly, Makayla N (7)
|
PVNCAP |
|
1:17.33 |
- |