| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Donoghue, Rowan B (11)
|
MADST |
56.84 |
56.46 |
-0.38 |
| 2 |
Dworak, Andrea M (11)
|
PVRMSC |
58.21 |
58.09 |
-0.12 |
| 3 |
Wu, Jingjing (11)
|
PVRMSC |
59.36 |
58.40 |
-0.96 |
| 4 |
Schwieters, Julie K (11)
|
PVRMSC |
1:00.28 |
59.59 |
-0.69 |
| 5 |
Daniel, Lucy A (11)
|
PVOCCS |
1:00.13 |
1:00.68 |
0.55 |
| 6 |
Bartholomew, Isla (11)
|
PVRMSC |
1:01.21 |
1:00.77 |
-0.44 |
| 7 |
DeBaecke, Emerson G (11)
|
MADST |
1:03.50 |
1:01.36 |
-2.14 |
| 8 |
Stabler, Ainsley (11)
|
PVOCCS |
1:02.96 |
1:03.22 |
0.26 |
| 9 |
Picardo, Nina Karylle B (11)
|
PVOCCS |
1:04.30 |
1:03.42 |
-0.88 |
| 10 |
Luetkenhaus, Caroline E (11)
|
PVOCCS |
1:05.62 |
1:03.91 |
-1.71 |
| 11 |
Phillips, Alexandra J (11)
|
MADST |
1:03.10 |
1:06.37 |
3.27 |