| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Colborn, Georgia A (12)
|
MADST |
59.32 |
56.94 |
-2.38 |
| 2 |
Horning, Caitlin L (12)
|
MADST |
57.36 |
56.99 |
-0.37 |
| 3 |
Carr, Hannah G (12)
|
PVRMSC |
1:01.68 |
1:01.12 |
-0.56 |
| 4 |
Noh, Ellie K (12)
|
PVRMSC |
1:05.12 |
1:05.50 |
0.38 |
| 5 |
Grijalba, Isabella (12)
|
PVRMSC |
1:06.62 |
1:05.83 |
-0.79 |
| 6 |
Caponiti, Adrianna L (12)
|
PVRMSC |
1:06.87 |
1:06.14 |
-0.73 |
| 7 |
Ruszala, Hailey C (12)
|
MADST |
1:07.29 |
1:06.60 |
-0.69 |
| 8 |
Brady, Peyton S (12)
|
PVOCCS |
1:07.45 |
1:07.28 |
-0.17 |
| 9 |
Carfi, Victoria (12)
|
MADST |
1:06.19 |
1:08.59 |
2.40 |
| 10 |
Petrazzuolo-Leiva, Chiara M (12)
|
PVOCCS |
1:14.62 |
1:12.50 |
-2.12 |
| 11 |
Hannam, Charlotte Q (12)
|
PVOCCS |
1:10.91 |
1:13.85 |
2.94 |
| 12 |
Worth, Brooke M (12)
|
PVOCCS |
1:15.13 |
1:13.96 |
-1.17 |