| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harms, Stella J (7)
|
PVNCAP |
49.05 |
44.22 |
-4.83 |
| 2 |
Cypher, Fiona S (7)
|
PVNCAP |
|
49.95 |
- |
| 3 |
Dunn, Olive M (6)
|
PVNCAP |
|
53.65 |
- |
| 4 |
Dufault, Audrey A (7)
|
PVNCAP |
|
53.92 |
- |
| 5 |
Cho, Olivia Y (6)
|
PVMACH |
57.69 |
54.82 |
-2.87 |
| 6 |
Moss, Kaitlin A (7)
|
PVCSC |
|
1:06.87 |
- |
| 7 |
Jung, Alina Y (7)
|
PVNCAP |
|
1:09.66 |
- |
| 8 |
Smith, Tatum (6)
|
PVYASD |
|
1:09.76 |
- |
| 9 |
Cho, Claire Y (6)
|
PVMACH |
1:11.83 |
1:17.88 |
6.05 |
| 10 |
Kissner-Ventimglia, Lucia (6)
|
PVYASD |
|
1:25.87 |
- |
|
Devasahayam, Deborah S (7)
|
PVNCAP |
|
DQ |
- |
|
Lucchese, Alexandra G (7)
|
PVNCAP |
1:08.34 |
DQ |
- |
|
Shive, Keira X (7)
|
PVNCAP |
59.66 |
NS |
- |