| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Luo, Angelina K (6)
|
PVRMSC |
22.77 |
24.29 |
1.52 |
| 2 |
Pezzimenti, Natalia M (6)
|
PVMACH |
28.93 |
28.39 |
-0.54 |
| 3 |
Waterman, Emma M (6)
|
PVRMSC |
33.27 |
28.69 |
-4.58 |
| 4 |
Busen, McKinley R (6)
|
PVCSC |
29.82 |
28.89 |
-0.93 |
| 5 |
Shi, Emma X (6)
|
PVRMSC |
35.42 |
30.90 |
-4.52 |
| 6 |
Bhardwaj, Adya (6)
|
PVASA |
33.88 |
31.13 |
-2.75 |
| 7 |
Groves, Charlotte (6)
|
PVASA |
31.04 |
31.47 |
0.43 |
| 8 |
Wofford, Elise J (6)
|
PVRMSC |
32.33 |
32.59 |
0.26 |
| 9 |
Jarrin, Iris H (6)
|
PVDCPR |
44.90 |
33.71 |
-11.19 |
| 10 |
Kuzmenko, Alina M (6)
|
PVRMSC |
36.49 |
34.30 |
-2.19 |
| 11 |
Duan, Jacy H (6)
|
PVRMSC |
36.83 |
35.28 |
-1.55 |
| 12 |
Peterson, Grace S (6)
|
PVRMSC |
39.28 |
36.22 |
-3.06 |
| 13 |
McConnell, Caroline A (5)
|
PVRMSC |
|
44.58 |
- |
|
Chen, Zella (6)
|
PVRMSC |
|
DQ |
- |
|
Wang, Audrey J (6)
|
PVRMSC |
37.17 |
DQ |
- |
|
Buckner, Zora N (6)
|
PVDCPR |
|
DQ |
- |
|
Bell, Ainsley C (6)
|
PVASA |
|
DQ |
- |
|
Kim, Katie J (6)
|
PVRMSC |
|
NS |
- |
|
Bartley, Leah K (6)
|
PVRMSC |
|
NS |
- |