| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaston, Aleah S (7)
|
PVNCAP |
46.92 |
41.58 |
-5.34 |
| 2 |
MacIvor, Amelia F (7)
|
PVCSC |
45.25 |
41.97 |
-3.28 |
| 3 |
Munchel, Dylan R (7)
|
PVPM |
42.46 |
42.99 |
0.53 |
| 4 |
Dressendorfer, Carson E (7)
|
PVMACH |
|
52.60 |
- |
| 5 |
Trinh, Teralynn T (7)
|
PVPM |
53.75 |
53.25 |
-0.50 |
| 6 |
Eastman, Ella E (7)
|
PVPM |
51.31 |
53.76 |
2.45 |
| 7 |
Dressendorfer, Beatrice L (7)
|
PVMACH |
|
54.18 |
- |
| 8 |
Fox, Chloe (7)
|
PVYASD |
50.31 |
54.29 |
3.98 |
| 9 |
Sarathy, Anoushka (7)
|
PVMACH |
56.25 |
55.30 |
-0.95 |
| 10 |
Wang, Alice L (7)
|
PVFISH |
|
55.36 |
- |
| 11 |
Stark Puglisi, Chloe G (7)
|
PVPM |
56.90 |
55.89 |
-1.01 |
| 12 |
Davis, Julia (7)
|
PVYASD |
54.66 |
58.58 |
3.92 |
| 13 |
Dressendorfer, Ellis C (7)
|
PVMACH |
|
1:04.39 |
- |
| 14 |
Jones-Puthoff, Daniella L (7)
|
PVFAST |
1:09.99 |
1:21.94 |
11.95 |
|
Almeida, Veronica (7)
|
PVPM |
|
NS |
- |
|
Bui, Emma T (7)
|
PVMACH |
45.90 |
NS |
- |
|
Lee, Alyson (7)
|
PVFISH |
1:01.09 |
NS |
- |
|
Clark, Keegan E (7)
|
PVCSC |
|
NS |
- |