| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shuler, Sofia A (13)
|
SRSA |
30.53 |
30.82 |
0.29 |
| 2 |
Lund, Jaycee D (14)
|
SRMVM |
36.25 |
34.36 |
-1.89 |
| 3 |
Derderian, Kate M (14)
|
SRBSC |
35.46 |
34.69 |
-0.77 |
| 4 |
Fast, Paige A (13)
|
SRBY |
37.88 |
35.85 |
-2.03 |
| 5 |
Hanson, Sofia M (13)
|
SRSA |
37.01 |
36.09 |
-0.92 |
| 6 |
Darling, Madeleine R (14)
|
UTPCS |
|
39.01 |
- |
| 7 |
Christensen, Lilian M (14)
|
SRNST |
50.15 |
40.29 |
-9.86 |
| 8 |
McCrory, Evalina J (13)
|
SRBY |
53.35 |
40.33 |
-13.02 |
| 9 |
Bergseng, Julia F (13)
|
SRBY |
|
43.58 |
- |
| 10 |
Jagger, Lindsey A (14)
|
SRBY |
|
47.31 |
- |
|
Bytwerk, Zoe R (13)
|
SRBSC |
37.12 |
DQ |
- |