| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Isabelle T (10)
|
GULIFE |
41.56 |
37.17 |
-4.39 |
| 2 |
Tilley, Katherine A (10)
|
GULIFE |
47.13 |
42.05 |
-5.08 |
| 3 |
Haik, Olivia K (10)
|
GUSTA |
46.64 |
43.60 |
-3.04 |
| 4 |
Ferrero D'Ambrosio, Kira (10)
|
GUTTST |
51.51 |
45.76 |
-5.75 |
| 5 |
Slattery, Eesha (10)
|
GULIFE |
52.12 |
45.91 |
-6.21 |
| 6 |
Andrade, Mia A (10)
|
GUSSS |
53.64 |
46.15 |
-7.49 |
| 7 |
Gimenez, Lucia B (10)
|
GULIFE |
|
47.91 |
- |
| 8 |
Ferrero D'Ambrosio, Dana (9)
|
GUTTST |
57.95 |
49.68 |
-8.27 |
| 9 |
Taylor, Lily A (10)
|
GUSTA |
|
49.74 |
- |
| 10 |
Lopez, Jocelyne (10)
|
GUAMBU |
|
50.11 |
- |
| 11 |
Harris, Trinity L (10)
|
GUSSS |
52.40 |
50.73 |
-1.67 |
| 12 |
Tung, Angelina (10)
|
GUTTST |
54.62 |
51.02 |
-3.60 |
| 13 |
Sustala, Delaney (10)
|
GUSSS |
1:00.42 |
51.55 |
-8.87 |
| 14 |
Harris, Sofia R (10)
|
GULIFE |
|
52.26 |
- |
| 15 |
Moultrie, Alden S (10)
|
GUTTST |
56.65 |
53.78 |
-2.87 |
| 16 |
Luedde, Katie A (9)
|
GUSSS |
|
54.59 |
- |
| 17 |
Dugas, Kyla G (10)
|
GUSTA |
|
55.43 |
- |
| 18 |
Aly, Samantha G (10)
|
GULIFE |
|
55.93 |
- |
| 19 |
Constantinides, Maria (10)
|
GULIFE |
|
56.26 |
- |
| 20 |
Heras, Alexandra (10)
|
GULIFE |
|
57.65 |
- |
| 21 |
Birkhoff, Sandra (9)
|
GULIFE |
1:05.94 |
1:00.44 |
-5.50 |
| 22 |
Olalde, Neva (9)
|
GUSSS |
1:02.89 |
1:00.66 |
-2.23 |
| 23 |
Shadow, Hailey R (10)
|
GUAMBU |
|
1:01.07 |
- |
| 24 |
Baptiste, Dyllen M (9)
|
GULIFE |
|
1:03.00 |
- |
| 25 |
Brown, Alyssa (10)
|
GUEPRC |
|
1:06.29 |
- |
| 26 |
Denney, Marisa L (9)
|
GUAMBU |
|
1:07.45 |
- |
|
Pruneda, Melania G (9)
|
GUSSS |
|
DQ |
- |
|
Medellin, Mackenzie K (10)
|
GUSSS |
|
DQ |
- |
|
Barraza, Leah (10)
|
GULIFE |
58.29 |
DQ |
- |
|
Hammett, Ireland J (9)
|
GULIFE |
|
DQ |
- |
|
Ashby, Bryana N (10)
|
GUAMBU |
|
DQ |
- |