| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chiarotto, Joanne O (17)
|
NIUN-D |
50.27 |
54.91 |
4.64 |
| 2 |
Steins, Kristina (23)
|
NIUN-D |
53.00 |
56.54 |
3.54 |
| 3 |
Barros, Debora (15)
|
NIUN-B |
56.79 |
59.42 |
2.63 |
| 4 |
Herman, Olivia T (16)
|
NITWST |
57.49 |
1:01.71 |
4.22 |
| 5 |
Fortna, Julia M (16)
|
NIHHSC |
1:01.77 |
1:03.11 |
1.34 |
| 6 |
Fortna, Jane E (16)
|
NIHHSC |
1:01.92 |
1:04.48 |
2.56 |
| 7 |
Hodge, Emily E (16)
|
NITWST |
1:02.79 |
1:04.98 |
2.19 |
| 8 |
Musterait, Madeline A (15)
|
NITWST |
1:03.62 |
1:07.73 |
4.11 |
| 9 |
Aldrich, Gabrielle E (16)
|
NIHHSC |
1:05.65 |
1:07.82 |
2.17 |
| 10 |
Lehnen, Kirsten M (16)
|
NIHAMB |
|
1:09.37 |
- |
| 11 |
Bement, Emily A (16)
|
NIHAMB |
1:10.17 |
1:09.54 |
-0.63 |
| 12 |
Dynas, Gabriella F (15)
|
NITWST |
|
1:11.28 |
- |
| 13 |
Regan, Abby S (15)
|
NITWST |
1:16.01 |
1:17.32 |
1.31 |
| 14 |
Kerr, Aurora A (15)
|
NIPVSC |
1:15.89 |
1:25.50 |
9.61 |
| 15 |
Carlson, Renee T (15)
|
NIEAST |
1:33.79 |
1:37.26 |
3.47 |