| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Black, Erynn A (17)
|
SCRAYS |
30.75 |
32.74 |
1.99 |
| 2 |
Pennington, Isabel K (15)
|
NCTYDE |
31.66 |
32.93 |
1.27 |
| 5 |
Newnam, Anna S (18)
|
NCWNCY |
33.56 |
34.88 |
1.32 |
| 6 |
Schlitt, Kayla M (16)
|
NCWNCY |
33.29 |
34.89 |
1.60 |
| 7 |
Sutter, Olivia C (16)
|
NCWNCY |
34.99 |
35.01 |
0.02 |
| 8 |
Ross, Christina N (15)
|
NCLOY |
35.12 |
35.03 |
-0.09 |
| 9 |
Klosterman, Carstyn J (15)
|
NCTYDE |
35.26 |
35.25 |
-0.01 |
| 10 |
Horan, Kathryn K (16)
|
SCYCSC |
33.88 |
35.78 |
1.90 |
| 12 |
Arwood, Fiona C (15)
|
NCWNCY |
36.83 |
37.75 |
0.92 |
| 13 |
Parks, Elizabeth N (17)
|
NCTYDE |
37.17 |
37.77 |
0.60 |
| 14 |
Morgan, Victoria M (17)
|
SCRAYS |
40.82 |
38.78 |
-2.04 |
| 15 |
Davis, Jessica C (17)
|
NCTYDE |
37.16 |
39.42 |
2.26 |
| 16 |
Culp, Brenna E (16)
|
NCRACY |
39.78 |
39.90 |
0.12 |
| 19 |
Baruth, Paige (15)
|
NCHY |
44.79 |
45.73 |
0.94 |
|
Reeder, Grace C (17)
|
NCWNCY |
31.84 |
NS |
- |
|
Bernesser, Skylar H (15)
|
SCRAYS |
44.27 |
NS |
- |