| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Whitworth, Nathan C (14)
|
NCTYDE |
38.03 |
32.87 |
-5.16 |
| 2 |
Thompson, Simon B (13)
|
SCYCSC |
31.41 |
33.26 |
1.85 |
| 3 |
Yeh, Alexander T (14)
|
NCLOY |
34.10 |
33.76 |
-0.34 |
| 4 |
Insixiengmay, Steven (14)
|
NCTYDE |
38.97 |
35.20 |
-3.77 |
| 5 |
Claborn, Nathan M (14)
|
NCLOY |
36.21 |
35.44 |
-0.77 |
| 6 |
Lucktong, Samuel P (14)
|
NCLOY |
35.45 |
35.50 |
0.05 |
| 7 |
Prince, Wyatt (14)
|
SCGYG |
33.67 |
37.06 |
3.39 |
| 8 |
Stoup, Kenan J (13)
|
NCLOY |
38.99 |
37.75 |
-1.24 |
| 9 |
Vess, Jack J (13)
|
NCWNCY |
37.65 |
38.83 |
1.18 |
| 10 |
Cassidy, Austin W (14)
|
NCTYDE |
42.60 |
39.32 |
-3.28 |
| 11 |
Seidel, Tyler W (13)
|
NCLOY |
39.50 |
40.09 |
0.59 |
| 13 |
Hartsell, David A (13)
|
NCLOY |
42.92 |
43.80 |
0.88 |
| 15 |
Orndorff, Evan M (13)
|
NCTYDE |
55.99 |
46.85 |
-9.14 |
| 16 |
Voyles, Brady (13)
|
SCGYG |
49.29 |
47.50 |
-1.79 |
| 17 |
Bell, Jacob M (13)
|
NCTYDE |
46.99 |
57.24 |
10.25 |
|
MacDermott, Connor E (14)
|
NCRACY |
44.47 |
DQ |
- |