| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kendall, Logan F (10)
|
NIMERC |
46.24 |
44.39 |
-1.85 |
| 2 |
Kelley, Cohen R (10)
|
NIGRAT |
50.70 |
45.97 |
-4.73 |
| 3 |
Lewis, Christopher J (10)
|
NIQUIK |
48.20 |
49.38 |
1.18 |
| 4 |
Kreiley, Jacob G (9)
|
NIMERC |
50.60 |
49.43 |
-1.17 |
| 5 |
Ray, Landon M (8)
|
NIVICT |
51.55 |
50.96 |
-0.59 |
| 6 |
Eagley, Wyatt T (10)
|
NIPMSC |
50.45 |
51.29 |
0.84 |
| 7 |
Stahl, Reed P (10)
|
NIVICT |
49.55 |
52.10 |
2.55 |
| 8 |
Battaglia, Benjamin P (9)
|
NIVICT |
55.61 |
52.37 |
-3.24 |
| 9 |
Iasnik, Noah V (10)
|
NICSCI |
53.89 |
53.13 |
-0.76 |
| 10 |
Griffin, Louis B (9)
|
NIPMSC |
|
54.73 |
- |
| 11 |
Shields, Zachary W (10)
|
NIVICT |
51.62 |
57.36 |
5.74 |
| 12 |
Rene, Jax R (9)
|
NIIRON |
1:00.40 |
58.63 |
-1.77 |
| 13 |
Wojieck, Mason G (10)
|
NIWGSC |
1:04.51 |
1:01.00 |
-3.51 |
| 14 |
Ashby, Colton J (6)
|
NISDSC |
1:04.64 |
1:06.83 |
2.19 |
| 15 |
Krul, Landon C (9)
|
NIWGSC |
1:20.13 |
1:32.86 |
12.73 |
|
Gohringer, Mason J (9)
|
NIIRON |
|
DQ |
- |
|
Simizon, Elijah K (10)
|
NIGRAT |
|
NS |
- |