| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Regelsberger, Emily C (15)
|
NIVICT |
55.40 |
56.28 |
0.88 |
| 2 |
Ritz, Erin (16)
|
NIVICT |
54.82 |
56.95 |
2.13 |
| 3 |
Guagliardo, Gabriella N (17)
|
NIIRON |
57.75 |
57.97 |
0.22 |
| 4 |
Branch, Alison G (16)
|
NIIRON |
58.53 |
58.48 |
-0.05 |
| 5 |
Thurston, Sophia R (17)
|
NIIRON |
59.30 |
59.30 |
0.00 |
| 6 |
Berkhof, Lucia M (17)
|
NIVICT |
55.01 |
59.56 |
4.55 |
| 7 |
Eaton, Paige M (15)
|
NIVICT |
57.39 |
1:00.18 |
2.79 |
| 8 |
Moore, Madison M (15)
|
NIVICT |
59.27 |
1:01.11 |
1.84 |
| 9 |
Snyder, Danielle E (16)
|
NICAQY |
1:02.34 |
1:01.37 |
-0.97 |
| 10 |
Christodaro, Melina A (16)
|
NIHAST |
59.72 |
1:02.88 |
3.16 |
| 11 |
Neubecker, Sarah C (17)
|
NICAQY |
1:02.28 |
1:04.30 |
2.02 |
| 12 |
Chadwick, Alina V (15)
|
NISDSC |
1:07.99 |
1:07.76 |
-0.23 |
| 13 |
Davison, Asella M (15)
|
NISDSC |
1:11.75 |
1:12.95 |
1.20 |