| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Park, Rebecca (14)
|
NIVICT |
25.82 |
26.21 |
0.39 |
| 2 |
Hawkins, Emma (14)
|
NIVICT |
26.62 |
27.69 |
1.07 |
| 3 |
Pathammavong, Suriyathai A (13)
|
NIVICT |
27.30 |
28.17 |
0.87 |
| 4 |
Senglaub, Katie (14)
|
NIVICT |
28.70 |
28.24 |
-0.46 |
| 5 |
Patterson, Ellen R (14)
|
NIIRON |
28.76 |
28.75 |
-0.01 |
| 6 |
Tytler, Candace L (13)
|
NIIRON |
31.02 |
29.46 |
-1.56 |
| 7 |
Hogan, Rachel L (13)
|
NICAQY |
30.62 |
30.72 |
0.10 |
| 8 |
Wallace, Emma E (14)
|
NIGRAT |
29.25 |
30.85 |
1.60 |
| 9 |
Caldwell, Hannah M (12)
|
NIVICT |
30.68 |
30.88 |
0.20 |
| 10 |
Natemeier, Amelia E (11)
|
NIVICT |
30.19 |
31.27 |
1.08 |
| 11 |
Martin, Lauren G (12)
|
NISDSC |
30.94 |
31.66 |
0.72 |
| 12 |
Cassidy, Alexa E (13)
|
NISDSC |
33.53 |
32.39 |
-1.14 |
| 13 |
Cywinski, Anna C (10)
|
NIVICT |
32.85 |
32.46 |
-0.39 |
| 14 |
McFarland, Emily S (14)
|
NICAQY |
31.28 |
33.18 |
1.90 |
| 15 |
Mullen, Nataelee A (13)
|
NICAQY |
33.35 |
33.21 |
-0.14 |
| 16 |
Werner, Riley K (13)
|
NIVICT |
32.32 |
33.45 |
1.13 |
| 17 |
Prezyna, Zoey (10)
|
NIVICT |
31.49 |
33.62 |
2.13 |
| 18 |
Christodaro, Raena M (13)
|
NIHAST |
33.07 |
33.83 |
0.76 |
| 19 |
Robinson, Sara C (11)
|
NISDSC |
33.64 |
34.03 |
0.39 |
| 20 |
Schwandt, Natalie K (10)
|
NIGRAT |
33.10 |
34.40 |
1.30 |
| 21 |
Bennett, Olivia R (11)
|
NICAQY |
34.17 |
34.56 |
0.39 |
|
Kazmark, Raelyn J (13)
|
NIVICT |
30.07 |
NS |
- |
|
Bolte-Pauley, Ashlyn R (14)
|
NIGRAT |
|
NS |
- |