| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
41.35 |
41.88 |
0.53 |
| 2 |
Hughes, Samantha G (12)
|
NICLSC |
41.73 |
42.42 |
0.69 |
| 3 |
Natemeier, Amelia E (11)
|
NIVICT |
49.13 |
45.78 |
-3.35 |
| 4 |
Guagliardo, Giovanna (12)
|
NIIRON |
48.47 |
46.68 |
-1.79 |
| 5 |
Kaiser, Grace (12)
|
NIAMTS |
|
49.96 |
- |
| 6 |
Ozbas, Melek (11)
|
NIWEBS |
51.00 |
50.10 |
-0.90 |
| 7 |
Huerta-Andrade, Isabella (12)
|
NIPACK |
54.91 |
52.56 |
-2.35 |
| 8 |
Hersh, Erin (11)
|
NIVICT |
1:13.90 |
52.58 |
-21.32 |
| 9 |
Munson, Sophie M (11)
|
NIGRSC |
57.88 |
56.50 |
-1.38 |
| 10 |
Resch, Rebecka J (11)
|
NIGRSC |
57.24 |
59.53 |
2.29 |
|
Romano, Bria M (12)
|
NINEWW |
54.75 |
DQ |
- |
|
Hubbard, Mary Elena P (12)
|
NIGRSC |
59.79 |
NS |
- |
|
Hicks, Rebecca N (11)
|
NIPACK |
51.27 |
NS |
- |