| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sokolowski, Suzanne N (14)
|
NIWEBS |
35.46 |
33.91 |
-1.55 |
| 2 |
Ritter, Anne (14)
|
NIPACK |
32.68 |
35.45 |
2.77 |
| 3 |
Hahn, Elizabeth P (14)
|
NIWEBS |
39.34 |
37.56 |
-1.78 |
| 4 |
Shaw, Kathryn E (13)
|
NIWEBS |
39.39 |
37.88 |
-1.51 |
| 5 |
Wolf, Taylor E (14)
|
NIAMTS |
|
41.11 |
- |
| 6 |
VanHeyningen, Katrina J (14)
|
NIWEBS |
55.00 |
42.61 |
-12.39 |
| 7 |
Werner, Riley K (13)
|
NIVICT |
52.44 |
43.03 |
-9.41 |
| 8 |
Manjerovic, Anna M (13)
|
NIPACK |
54.62 |
44.91 |
-9.71 |
| 9 |
Langlotz, Olivia M (14)
|
NICLSC |
45.07 |
45.61 |
0.54 |
|
Faller, Karsen E (13)
|
NIAMTS |
48.47 |
NS |
- |
|
Miller, Anna M (13)
|
NIGRSC |
39.49 |
NS |
- |
|
Thompson, Daniella J (13)
|
NIGRSC |
51.07 |
NS |
- |
|
Williams, Tory L (13)
|
NIPACK |
48.47 |
NS |
- |