| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cropper, Iala K (13)
|
NIFAST |
39.65 |
40.27 |
0.62 |
| 2 |
Sokolowski, Suzanne N (14)
|
NIWEBS |
37.47 |
40.42 |
2.95 |
| 3 |
Shaw, Kathryn E (13)
|
NIWEBS |
41.75 |
41.80 |
0.05 |
| 4 |
Manjerovic, Anna M (13)
|
NIPACK |
43.40 |
43.41 |
0.01 |
| 5 |
Hahn, Elizabeth P (14)
|
NIWEBS |
44.42 |
45.21 |
0.79 |
| 6 |
Smillie, Eleanor B (14)
|
NIPACK |
50.91 |
47.34 |
-3.57 |
| 7 |
VanHeyningen, Katrina J (14)
|
NIWEBS |
47.61 |
49.09 |
1.48 |
| 8 |
Langlotz, Olivia M (14)
|
NICLSC |
49.38 |
51.65 |
2.27 |
|
Faller, Karsen E (13)
|
NIAMTS |
43.00 |
NS |
- |
|
Thompson, Daniella J (13)
|
NIGRSC |
1:07.90 |
NS |
- |
|
Williams, Tory L (13)
|
NIPACK |
49.27 |
NS |
- |