| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeHollander, Erin K (12)
|
NIVICT |
5:16.96 |
4:55.80 |
-21.16 |
| 2 |
El Ghissassi, Ritaj (12)
|
NIVEST |
5:10.65 |
5:06.47 |
-4.18 |
| 3 |
Xu, Ming Na (12)
|
NIFAST |
5:17.52 |
5:11.46 |
-6.06 |
| 4 |
Adamczyk, Gabrielle J (12)
|
NICLSC |
5:20.72 |
5:16.59 |
-4.13 |
| 5 |
Dinh, Nakisha V (12)
|
NIWEBS |
5:37.45 |
5:17.68 |
-19.77 |
| 6 |
Caldwell, Hannah M (12)
|
NIVICT |
5:50.31 |
5:27.28 |
-23.03 |
| 7 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
5:36.66 |
5:38.56 |
1.90 |
| 8 |
McKinney, Madison N (11)
|
NICLSC |
5:38.00 |
5:45.44 |
7.44 |
| 9 |
Mintz, Vanessa M (12)
|
NICLSC |
5:40.13 |
5:58.64 |
18.51 |
| 10 |
Beutner, Ella R (12)
|
NIQUIK |
5:53.69 |
6:00.82 |
7.13 |
| 11 |
Pierce, Marek J (12)
|
NINEWW |
6:08.18 |
6:11.71 |
3.53 |
| 12 |
Onze, Emily S (11)
|
NIVICT |
7:11.03 |
6:26.95 |
-44.08 |
| 13 |
McRae, Kathryn E (12)
|
NIQUIK |
|
6:27.14 |
- |
| 14 |
Seelig, Jenna N (11)
|
NICLSC |
7:16.54 |
6:43.17 |
-33.37 |
| 15 |
Smith, Aubrey D (11)
|
NIWEBS |
|
7:22.63 |
- |
|
Shen, Alivia (11)
|
NIPACK |
5:27.89 |
NS |
- |
|
Huerta-Andrade, Isabella (12)
|
NIPACK |
6:38.20 |
NS |
- |
|
Griffin, Zoey O (12)
|
NICLSC |
5:19.30 |
NS |
- |