| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeHollander, Erin K (12)
|
NIVICT |
29.81 |
30.44 |
0.63 |
| 2 |
Flanigen, Ruby J (11)
|
NIIRON |
32.82 |
32.47 |
-0.35 |
| 3 |
Hogan, Meredith G (11)
|
NIVICT |
34.58 |
32.98 |
-1.60 |
| 4 |
McRae, Kathryn E (12)
|
NIQUIK |
35.60 |
33.52 |
-2.08 |
| 5 |
Guagliardo, Giovanna (12)
|
NIIRON |
33.09 |
33.53 |
0.44 |
| 6 |
Caldwell, Hannah M (12)
|
NIVICT |
35.53 |
34.24 |
-1.29 |
| 7 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
35.30 |
34.46 |
-0.84 |
| 8 |
Spencer, Elyse (11)
|
NIAMTS |
34.12 |
34.80 |
0.68 |
| 9 |
Wang, Floria M (11)
|
NIFAST |
38.50 |
34.98 |
-3.52 |
| 10 |
Carey, Madison H (12)
|
NIIRON |
36.14 |
35.52 |
-0.62 |
| 11 |
Natemeier, Amelia E (11)
|
NIVICT |
37.31 |
35.62 |
-1.69 |
| 12 |
Kaiser, Grace (12)
|
NIAMTS |
|
35.81 |
- |
| 13 |
Hersh, Erin (11)
|
NIVICT |
38.24 |
36.31 |
-1.93 |
| 14 |
Onze, Emily S (11)
|
NIVICT |
39.50 |
37.47 |
-2.03 |
| 15 |
Magnera, Sofia A (12)
|
NIWEBS |
39.92 |
40.20 |
0.28 |
| 16 |
Resch, Rebecka J (11)
|
NIGRSC |
39.82 |
40.43 |
0.61 |
| 17 |
Farina, Samantha R (11)
|
NIGRSC |
43.80 |
41.32 |
-2.48 |
| 18 |
Stefanou, Ava C (12)
|
NIQUIK |
40.70 |
44.48 |
3.78 |
| 19 |
Jones, Lillian M (12)
|
NIFAST |
43.42 |
44.57 |
1.15 |
| 20 |
Romano, Bria M (12)
|
NINEWW |
41.93 |
44.70 |
2.77 |
|
Hubbard, Mary Elena P (12)
|
NIGRSC |
36.94 |
NS |
- |