| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carlson, Avery M (8)
|
VADC |
48.42 |
43.20 |
-5.22 |
| 2 |
Tingen, Bailey S (8)
|
VAWAC |
|
44.01 |
- |
| 3 |
Durrette, Sierra C (8)
|
VARACE |
|
44.21 |
- |
| 4 |
Lobach, Mya M (8)
|
VASEVA |
49.00 |
45.72 |
-3.28 |
| 5 |
Vandermeyden, Grace E (8)
|
VASEVA |
47.71 |
47.75 |
0.04 |
| 6 |
Warren, Surai B (8)
|
VARACE |
50.86 |
49.16 |
-1.70 |
| 7 |
Robertson, Caroline C (7)
|
VAWAC |
|
51.95 |
- |
| 8 |
Crane, Berkeley M (8)
|
VADC |
|
52.20 |
- |
| 9 |
Kawecki, Lillian C (8)
|
VASEVA |
53.58 |
53.07 |
-0.51 |
| 10 |
Vogel, Anna M (7)
|
VADC |
|
55.08 |
- |
| 11 |
Elias, Grace (6)
|
VASEVA |
|
57.72 |
- |
| 12 |
Gonzalez, Allison M (7)
|
VASEVA |
1:06.00 |
1:00.19 |
-5.81 |
| 13 |
Shipe, Sydney D (7)
|
VASEVA |
1:08.00 |
1:05.83 |
-2.17 |