| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gunsolus, Meredith K (8)
|
NISWAT |
45.77 |
42.54 |
-3.23 |
| 2 |
Crone, Kiersten A (8)
|
NISWAT |
41.91 |
43.79 |
1.88 |
| 3 |
Smith, Juliana M (8)
|
NISAAC |
47.50 |
45.03 |
-2.47 |
| 4 |
Bradt, Addison M (8)
|
NIGVST |
48.20 |
47.71 |
-0.49 |
| 5 |
Green, Ella A (8)
|
NIGVST |
50.69 |
48.18 |
-2.51 |
| 6 |
Chase, Taylor R (8)
|
NIFKVL |
46.17 |
48.24 |
2.07 |
| 7 |
Maul, Katelin G (8)
|
NISWAT |
1:07.56 |
51.99 |
-15.57 |
| 8 |
Budinger, Annabelle R (8)
|
NIGVST |
58.60 |
52.00 |
-6.60 |
| 9 |
Ground, Jaylee D (8)
|
NISAAC |
53.82 |
53.32 |
-0.50 |
| 10 |
Illig, Kelcie R (8)
|
NISWAT |
53.72 |
53.45 |
-0.27 |
| 11 |
Jankowski, LilaEve L (7)
|
NIGWST |
55.88 |
54.28 |
-1.60 |
| 12 |
Reed, Alexa R (8)
|
NISAAC |
1:05.26 |
56.44 |
-8.82 |
| 13 |
Owens, Lillian D (8)
|
NIFKVL |
57.59 |
58.46 |
0.87 |
| 14 |
Owens, Annabelle R (7)
|
NIFKVL |
1:04.75 |
58.53 |
-6.22 |
| 15 |
Beaudette, Janessa J (7)
|
NIAAAC |
1:00.57 |
1:01.44 |
0.87 |
| 16 |
Sullivan, Mya M (7)
|
NIFKVL |
1:04.04 |
1:01.78 |
-2.26 |
| 17 |
Cook, Kendra M (7)
|
NISWAT |
58.58 |
1:02.19 |
3.61 |
| 18 |
Dougherty, Regina A (7)
|
NIGVST |
1:15.52 |
1:03.77 |
-11.75 |
| 19 |
Castellano, Rebecca J (7)
|
NIGVST |
1:10.12 |
1:05.19 |
-4.93 |
| 20 |
Slocum, Molly M (7)
|
NIAAAC |
|
1:11.94 |
- |
| 20 |
Redeye, Brenda M (8)
|
NISAAC |
1:10.25 |
46.31 |
-23.94 |
| 21 |
Ploetz, Madalynn K (8)
|
NISWAT |
|
1:12.93 |
- |
| 22 |
Hirsch, McKenna L (6)
|
NIAAAC |
1:21.48 |
1:22.04 |
0.56 |
| 23 |
Yasurek, Rylee (7)
|
NIGWST |
1:27.86 |
1:22.06 |
-5.80 |
| 24 |
Haley, Delaney J (6)
|
NISAAC |
1:29.48 |
1:26.81 |
-2.67 |
| 25 |
Bova, Keira E (7)
|
NISAAC |
1:27.14 |
1:43.98 |
16.84 |
| 26 |
Kane, Amelia M (7)
|
NISWAT |
|
1:49.15 |
- |